CD8A and dry eye syndrome: To investigate whether MHC-I upregulation in KCs promotes inflammation through activation of epidermal CD8+ T cells, we injected H2-D1-expressing adeno-associated virus serotype 2 (AAV2 H2-D1) intracutaneously into mouse ear to increase H2-D1 expression in the epidermis followed by topical IMQ treatment (Supplementary Fig. S10h, i).