Furthermore, we evaluated the contribution of CD8+ T cells to colitis by administering specific anti-CD4 and anti-CD8α depleting antibodies before DSS induction, compared with anti-CD8α treatment, Lztr1-depleted mice following anti-CD4 or IgG isotype administration showed alleviated intestinal injuries (Supplementary Fig. S13m–p), providing additional evidence for the interactions between epithelial LZTR1 and CD8+ T cell activation in additional autoimmune disease model. Here, LZTR1 is linked to autoimmune disease.