RT-qPCR of both in vitro and in vivo tumor cells also revealed significant up-regulation of key enzymes involved in glycosphingolipid biosynthesis, including St3gal1, St3gal2, B4galt5, Sptlc2, and Ugcg, in A159V-mutated cells (Fig. 5G). This evidence concerns the gene SPTLC2 and neoplasm.