Consistent with the flow cytometry findings, scRNA-seq analyses revealed a diminished abundance of CD8+ effector T cells within the RAC1A159V IgG tumor compared to WT IgG tumor, while anti-PD1 significantly augmented CD8+ effector T cell populations in WT tumors but exerted a limited effect in RAC1A159V tumors (Fig. 3, C and D). This evidence concerns the gene CD8A and neoplasm.