Triple-positive IFNγ/IL-2/TNF cells (i.e., all three cytokines detected in the same SFU), total IFNγ, and total IL-2 (i.e., any SFU containing IFNγ or IL-2, respectively, alone or in combination with any other cytokine) yielded the highest frequency in both the TBD group (88%), in the TBI group (81%–84%), and the two TB groups combined (83%–85%) (Fig. 2B). This evidence concerns the gene TNF and tuberculosis.