The resultant virus, designated as ToCV‐p22‐dsRed, containing ToR1‐p22‐dsRed and ToR2 (RNA2), was capable of successfully infecting N. benthamiana, causing chlorosis symptoms and expressing the coat protein (CP) that was comparable to that observed in wild‐type ToCV‐BJ infections (Figure 2B). This evidence concerns the gene GOLPH3 and infection.