RNA-sequencing analysis of endometriosis-derived organoids exhibited differential gene expression compared to both healthy and patient endometrium-derived organoids, with altered levels of genes related to ECM-receptor interaction (COL3A1, FN1), PI3K-AKT signaling (IGF1, ITGB8), WNT signaling (CTNNA2, WNT11), hormonal response (PGR, HSD11B1), Hippo signaling (CTGF), adhesion/invasion (MMP2, SNAI2), and developmental regulation (HOXD8, HOXA9). Here, PIK3CA is linked to endometriosis.