The systemic metabolic effects induced by HFHSD were accompanied by increased plasma ALT (an indicator of liver damage), augmented hepatic TG content, liver histological abnormalities (steatosis, hepatocyte ballooning, and fibrosis), and a higher histological score for the severity of fatty liver disease (NAS), which recapitulated the advanced stages of MASLD. The gene discussed is GPT; the disease is neonatal abstinence syndrome.