PRNP and prion disease: Inoculation of Tg60 mice (S96-deer PrPC) with the 116AG isolate confirmed its classification as at least one distinct and novel CWD strain, as evidenced by host susceptibility and a PrPSc banding pattern distinct from that of H95+ strain—the only CWD strain previously reported to induce prion disease in Tg60 mice—while Wisc-1 fails to transmit in this model [120,152,153].