DHTKD1 and infection: The integrity of the infected cells at the early time post infection chosen for our harvest (Figure 1) and the size of our purified EV population rule out the presence of the large cytotoxic vesicles encapsulating mature virions described by Ran and colleagues in their vesicle preparations derived from the A549 cells infected with oncolytic Ad5 hTERTp-E1A at 48 h post infection when most cells were detached from the culture dishes [19].