EDEM1 and Zika virus infectious disease: Consistent with this idea, induction of ER stress and the IRE1/XBP1 axis by treatment with tunicamycin (Tn) revealed both a potent splicing of the XBP1 mRNA and the induction of Erdj4 and Edem1 transcripts; thus, confirming a partial activation of XBP1s during ZIKV infection (Figure 2C,D).