Considering the induction of ER stress during infection together with the critical role of IRE1 in ZIKV replication in human cell models such as HeLa and A549 cells [42,43], we evaluated the activation of IRE1α (hereafter referred as IRE1) endonuclease activity during ZIKV infection at 24 and 48 hpi in the C20 human microglia cell line. This evidence concerns the gene ERN1 and infection.