From the above-mentioned findings in uninfected physioxic HCT-8 cells, it can be concluded that under physioxic conditions, oleocanthal host cell pre-treatment for 24 h prior to infection resulted in host cells with diminished glutaminolysis activity, a status that was already proven as adverse for C. parvum replication, since glutaminase inhibitors effectively reduced infection rates [12]. The gene discussed is GLS; the disease is infection.