Similarly, in MCF-7 breast cancer cells, both compounds induced a pronounced increase in the Annexin V+/PI+ population (59.5% for 8 and 73.9% for 12; Figure 7E,F,H), accompanied by a smaller increase in Annexin V−/PI+ cells, suggestive of direct necrotic damage (Figure 7H). Here, ANXA5 is linked to breast cancer.