Long-term diabetes (≥15 years), poor glycemic control, insulin therapy, hypertension, heart disease, renal impairment, stroke, and the presence of gangrene and ischemia are other independent predictors of major lower limb amputation, according to a recent retrospective study conducted in Jordan involving patients with type 2 diabetes associated with diabetic foot syndrome [37,38]. This evidence concerns the gene INS and hypertensive disorder.