Moreover, the staining with anti-LC3, anti-GABARAPs, or anti-GATE-16 (a member of the GABARAP family) antibodies revealed defective autophagy maturation and impaired autophagic flux due to decreased levels of autophagy-related proteins and elevated levels of adaptor proteins, such as SQSTM1/p62, in MSA (Figure 3F) [25]. This evidence concerns the gene SQSTM1 and multiple system atrophy.