To confirm transcriptional changes at the protein level, we analyzed the expression of three markers by western blot: SLC7A5, a ferroptosis marker upregulated in all four cell lines; RRM2, a ferroptosis marker differentially regulated across MM and LUAD cell lines; and DLAT, a cuproptosis marker upregulated in all four cell lines. The gene discussed is SLC7A5; the disease is Miyoshi myopathy.