A subsequent in-depth analysis of monocytes identified three distinct subsets: classical monocytes (CD14+), non-classical monocytes (CD16+), and intermediate monocytes (CD14+, CD16+), as illustrated in Figure 3D. Following this, we analyzed the expression patterns of marker genes across these subsets, as shown in Figure 3E. The differences in the proportions of monocyte subsets between the B-ALL cohorts and the HC cohorts were evaluated using the Wilcoxon test. The gene discussed is CD14; the disease is acute lymphoblastic leukemia.