To study the impact of hsa-miR-125b-5p on MMP-2 expression, A549 lung cancer cells were transfected with either anti-miR-125b-5p or pre-miR-125b-5p (100 nM), followed by stimulation with S100A4 (1 μg/mL) or left unstimulated. Here, S100A4 is linked to lung cancer.