TGFB1 and breast cancer: Krizbai et al. (2015) [14] prepared activated conditioned medium (ACM) by heating CM from melanoma (B16/F10, A2058) and breast cancer (MCF-7, MDA-MB-231) cells at 80 °C for 10 min to activate latent TGF-β and applied it to rat brain endothelial cells (RBECs) to induce EndMT.