The LDA results revealed a significantly increased expression of iron metabolism-related genes ferritin heavy chain 1 (FTH1), transferrin receptor (TFRC), and Acyl-CoA synthetase long-chain family member 4 (ACSL4), while GPX4 expression was decreased in the glioma samples compared to the controls (Figure 1E). Here, ACSL4 is linked to central nervous system cancer.