MACROH2A1 and cancer: In contrast, mH2A1-deposition-associated genes that were unique to HeLa during metaphase showed additional enrichment in cell-cycle-function-related pathways, such as DNA replication, DNA repair, and chromosome segregation [36,37,38,39,40] (Figure 3B).These findings suggested that in cancer cells, mH2A1 might reinforce chromatin stability in critical genomic regions, thereby supporting rapid proliferation and maintaining genomic integrity during cell division.