In this study, we determined the modulation of NBCn1 through NBC inhibitor S0859 in the presence of hydrogen peroxide-mediated oxidative stress and the regulatory role of NRF2 and the relationship between NBCn1 and NRF2 in lung cancer cells to verify the cellular mechanism of NRF2-associated network and to support expanding the developmental anti-cancer strategy against NSCLC. Here, NFE2L2 is linked to cancer.