KRT8 and cancer: The established flow cytometry gating strategy, based on the analysis of cancer cell lines and healthy cervical biofluids, suggests that tumor-derived epithelial cells localize within both “population 1” and “population 2.” However, CK8 expression serves as a distinguishing feature, as CK8 positive cells within “population 1” are indicative of tumor origin, given that this population is CK8 negative in healthy samples (as shown above).