The established flow cytometry gating strategy, based on the analysis of cancer cell lines and healthy cervical biofluids, suggests that tumor-derived epithelial cells localize within both “population 1” and “population 2.” However, CK8 expression serves as a distinguishing feature, as CK8 positive cells within “population 1” are indicative of tumor origin, given that this population is CK8 negative in healthy samples (as shown above). This evidence concerns the gene KRT8 and neoplasm.