Furthermore, to investigate the role of TCN1 in pancreatic cancer progression, we established stable TCN1-knockdown (BxPC-3) and TCN1-overexpressing (PANC-1) cell lines through lentiviral infection, with successful modulation confirmed using qRT-PCR (Supplementary Figure S1A,B). This evidence concerns the gene TCN1 and familial pancreatic carcinoma.