An earlier GEMM of brainstem glioma by Becher and colleagues in 2010 using the RCAS/tv-a system was derived by injecting 1 μL (105) RCAS-PDGF-B-expressing DF1 cells into nestin tv-a (Ntv-a) or Ntv-a;Ink4a-ARF−/− mice [85]. The gene discussed is CDKN2A; the disease is brain stem glioma.