More recently, du Chatinier and colleagues developed an immunocompetent DMG mouse by first establishing primary tumor cell lines from murine DMG tumors generated by the intrauterine electroporation (IUE) of PiggyBac DNA plasmids and complementary PiggyBac transposases to introduce H3f3aK27M and Pdgfra mutations and dominant negative p53 into the fourth ventricles of embryonic day 13.5 pups of C57BL/6 immunocompetent mice [71]. Here, TP53 is linked to neoplasm.