ROBO1 variants associated with VSD (both in the membranous and muscular septum), ASD [450], malformation of the outflow tracts [214], TOF [451], BAV [452], overriding aorta, defects in canal veins [450], ascending aortic aneurysm [453] (human). Here, ROBO1 is linked to Ascending tubular aorta aneurysm.