To sum up, our study finds that PGRMC1 expression is elevated in the process of CIN-CSCC transformation, and the specific mechanism is that PGRMC1 binds to VIM protein, inhibits the phosphorylation level of VIM (Ser-39) site to maintain VIM assembly, and interferes with PGRMC1 expression to inhibit cervical cancer cell migration and invasion. Here, PGRMC1 is linked to cervical squamous intraepithelial neoplasia.