BRAF class I mutant tumors had significant lower median tumor mutation burden (4.8 mut/Mb vs. 8.65 mut/Mb Class II Wilcoxon p = 1.77e−9, 10.8 mut/Mb Class III Wilcoxon p < 10−10) and fraction genome altered when compared to class II and III (0.1 vs. 0.15 Class II Wilcoxon p = 0.021, 0.19 Class III Wilcoxon p = 9.31e−4 Supplemental Fig. 2). The gene discussed is BRAF; the disease is neoplasm.