Simulations were performed against the core targets identified through our multi-omics integrative approach: TP53, JUN, CASP3, and PTGS2 (from network toxicology); SIRT3 (the sole significant differentially expressed protein from transcriptomics); and PTK2 (a Mendelian randomization-validated causal protein for depression). This evidence concerns the gene SIRT3 and major depressive disorder.