Simulations were performed against the core targets identified through our multi-omics integrative approach: TP53, JUN, CASP3, and PTGS2 (from network toxicology); SIRT3 (the sole significant differentially expressed protein from transcriptomics); and PTK2 (a Mendelian randomization-validated causal protein for depression). The gene discussed is JUN; the disease is depressive symptom measurement.