Further to check the effect of E47 isoform on the invasion of breast cancer cells, we overexpressed FLAG-tagged TCF3-18A (E12) and TCF3-18B (E47) isoforms (Fig 6B and 6C) and performed Matrigel invasion assay in both MCF7 and MDA-MB231 cell lines. This evidence concerns the gene TCF3 and breast cancer.