To elucidate the regulatory role of the AhR in IBV infection, we assessed the effects of pharmacological modulation of AhR using its canonical antagonist CH223191 and agonist L-kynurenine (kynurenine) on in vitro infections with two IBV variants: the wild-type rIBV and the ncRNA-deficient mutant rIBV-C27107G. This evidence concerns the gene AHR and infection.