In our experiment, we used the human neuroblastoma cell line SH-SY5Y, after implementing a differentiation protocol based on retinoic acid and brain-derived neurotrophic factor (BDNF), in order to achieve cultured mature neurons, both in morphology and physiology, that have lengthened neurites and express mature neuronal markers [16,17]. The gene discussed is BDNF; the disease is neuroblastoma.