Moreover, reducing expression of nucleophosmin in cancer cells is known to induce apoptosis [47], explaining the observed induction of apoptosis when treating TNBC spheroids with aplysinamisine I. Nucleophosmin is also known to act upstream of the kinesin Eg5 to promote microtubule polymerization and to directly inhibit Eg5 ATPase activity to regulate microtubule dynamics [38]. This evidence concerns the gene NPM1 and cancer.