CALCA and persistent truncus arteriosus: After 15 days post-TAC, mice belonging to Sham+NMEG-CGRP (n = 7) and TAC+NMEG-CGRP (n = 9) groups began receiving subcutaneous injections of NMEG-CGRP (3.6 mg/kg/mouse) on alternate days over the course of 28 days (Figure 1A).