Similar to human renal cell carcinoma (RCC) 786-o and A498 cells, depletion of endogenous SPOP reduced colony formation in human melanoma A2058 and HMCB cells (Supplemental Figure 1, A and B; supplemental material available online with this article; https://doi.org/10.1172/JCI191772DS1). Here, SPOP is linked to hereditary clear cell renal cell carcinoma.