In order to see if our isolated EV fractions recapitulate the pathological changes occurring in Alzheimer's disease, in terms of dysregulation (upregulated) of total‐Tau and phosphorylated‐Tau (P‐Tau 181), we measured the levels of total‐Tau and its phosphorylated form by ELISA assays in our isolated EV fractions. The gene discussed is MAPT; the disease is early-onset autosomal dominant Alzheimer disease.