Hematoxylin-eosin staining more intuitively showed that lung metastasis in the shTRIM17-Luc group was significantly reduced, and the luciferase activity of shTRIM17-Luc cells was also significantly lower than that of shNeg-Luc cells, which was consistent with the reduction of metastasis burden, suggesting that silencing TRIM17 can inhibit lung metastasis of osteosarcoma (Fig. 8H, I). The gene discussed is TRIM17; the disease is osteosarcoma.