Extreme limiting dilution assays revealed that YTHDC2-KO1 and YTHDC2-KO3 polyclonal cells (with stronger knockout efficiency than YTHDC2-KO2; Fig. 2A) exhibited significantly higher tumor-initiating ability in 5637 cells under low-density conditions (Fig. 3C). The gene discussed is YTHDC2; the disease is neoplasm.