Furthermore, results of flow cytometry demonstrated that YTHDC2 knockout increased the ALDH1+ cells and CD133+ populations (markers for bladder cancer stem cells) in 5637-derived tumorspheres (Fig. 3E–H), while in T24, overexpression of YTHDC2 decreased the ratio of CD133+ subpopulation (Fig. 3I, J). This evidence concerns the gene PROM1 and urinary bladder carcinoma.