Subsequent immunofluorescence (IF) staining revealed intensified cytoplasmic ARL5B signals in KYSE150 and TE‐1 cells compared to Het‐1A (p < 0.01 and p < 0.001 respectively; Figure 1G), highlighting ARL5B overexpression in ESCC. Here, ARL5B is linked to esophageal squamous cell carcinoma.