In cellular models, parallel analyses using quantitative reverse transcription polymerase chain reaction  (qRT‐PCR) and western blotting in normal esophageal epithelial cell line Het‐1A versus four ESCC cell lines (TE‐1, KYSE150, ECA109, and KYSE510) demonstrated concordant upregulation of ARL5B at both transcriptional (p < 0.01) and translational levels (p < 0.01) in malignant cells (Figure 1E,F). The gene discussed is ARL5B; the disease is esophageal squamous cell carcinoma.