The expression of 7 hub genes were further verified to study the effect of cuproptosis on diabetic cardiomyopathy.The results showed 4 of them were differentially expressed(Fig 12, S3 File).They were Cyp1a1 (up),Hmgcs2 (up),IDH1 (up) and HK2 (down).The expression of Cyp1a1, Hmgcs2, IDH1 and HK2 were consistent with the predicted result. This evidence concerns the gene HMGCS2 and diabetic cardiomyopathy.