The expression of 7 hub genes were further verified to study the effect of cuproptosis on diabetic cardiomyopathy.The results showed 4 of them were differentially expressed(Fig 12, S3 File).They were Cyp1a1 (up),Hmgcs2 (up),IDH1 (up) and HK2 (down).The expression of Cyp1a1, Hmgcs2, IDH1 and HK2 were consistent with the predicted result. The gene discussed is CYP1A1; the disease is diabetic cardiomyopathy.