EIF2B4 and hepatocellular carcinoma: 1B). Wound healing assays revealed that EIF2B4 depletion markedly impaired the migratory capacity of HCC cells compared to the non-tumorigenic L02 cells (Fig. 4F). Based on Chronos dependency scores, Hep3B was identified as the most EIF2B4-dependent HCC cell line and was therefore selected for subsequent functional investigations (Fig. 4G) [21].