TGFB1 and neoplasm: Transcriptomic profiling further revealed elevated Cd8a and Ifng (markers of cytotoxic T cell infiltration/activity) alongside reduced Foxp3 (Tregs), Cd163 (M2-polarized tumor-associated macrophages), and Tgfb1 (immunosuppressive cytokine) in KO tumors (Fig. 6O).