However, GS overexpression should not be considered the defining characteristic of increased β-catenin activity, and many cases that do not exhibit atypical morphologic or clinical signs of HCC may show strong GS staining (29).In contrast, the combined use of other tissue markers such as GPC3, HSP70, glypican-3, and CD10 can further increase specificity and sensitivity (30). The gene discussed is APC; the disease is hepatocellular carcinoma.