Moreover, interaction analysis revealed that patients with both high PLOD expression and high tumor grade (Grade 3/4) had the poorest survival outcomes, whereas those with low/medium PLOD expression and low tumor grade (Grade 1/2) showed the most favorable prognosis (Figure 3C), underscoring the combined prognostic effect of PLOD expression and tumor grade. The gene discussed is PLOD1; the disease is neoplasm.