MYCN and neuroblastoma: We recruited 168 participants, including 24 children with NB (9 patients with MYCN+ HR, 8 patients with MYCN− HR, and 7 patients with MYCN− IR or LR) as well as 10 HCs in the discovery stage, 87 NB children (28 patients with MYCN+ HR, 33 patients with MYCN− HR, and 26 patients with MYCN− IR or LR), and 47 HCs during the validation phase in this study (Figure 1).