We conducted Western blot analysis to examine the effects of phillyrin on the PI3K/AKT signaling pathway in colorectal cancer cells by assessing the levels of PI3K, p‐PI3K, AKT, p‐AKT, mTOR, and p‐mTOR, along with their downstream targets, in HT29 and HCT116 cells. This evidence concerns the gene MTOR and colorectal cancer.