Furthermore, results of the qRT-PCR and WB analyses showed that the C1orf122 mRNA and protein levels in five HCC cell strains (HuH-7, HepG2, Hep3B, SMMC7721, MHCC-97H) were significantly higher than in normal hepatocytes (THLE-2) (Fig. 1D; Fig. S6B). This evidence concerns the gene C1orf122 and hepatocellular carcinoma.