Quantification of the images as described above confirmed that islet MMP-3 intensity multiplied by staining area normalized to insulin positive area was increased in Aab and T1D donors compared to healthy controls, where MMP-3 levels were highest in T1D donors that were presumed hyperglycemic (p<0.001, p<0.001, Figure 6A). This evidence concerns the gene INS and type 1 diabetes mellitus.