SIRT1 and obesity due to melanocortin 4 receptor deficiency: Under obese status (fixed obesity factor), a significant difference was also detected between the HSG and HEG [F(1,40) = 5.781; partial η2 = 0.162; P = 0.023], with the HEG showing significantly higher Sirt1 mRNA levels than the HSG (I-J = 0.706; P = 0.023).