To extend our studies to human AML, we used doxycycline-inducible CRISPR/Cas939,40 to delete MNT from 4 long-established human AML CLs, namely MV4;11, which harbors an MLL::AF4 fusion gene; MOLM-13 and THP-1, which have MLL::AF9 fusion genes; and OCI-AML3, which has the NPM1 and DNMT3A mutations that are common in adult AML.59 Here, AFF1 is linked to acute myeloid leukemia.