In a regional pilot study aiming to demonstrate the feasibility of using the assay as a Lynch syndrome urothelial carcinoma screening test, we screened urine samples from asymptomatic individuals with a diagnosis of MSH2-Lynch syndrome and unselected cases of UTUC to calculate initial estimates of the sensitivity and specificity of the assay for MMRd urothelial carcinoma detection. Here, MSH2 is linked to Lynch syndrome.