<h4>Objective</h4>We aimed to evaluate the clinical utility of serum neurofilament light chain (sNfL) and cardiac troponin T (cTnT) in amyotrophic lateral sclerosis (ALS) and assess whether their combination improves diagnostic accuracy.<h4>Methods</h4>We retrospectively analyzed 293 ALS patients, 85 neurodegenerative disease controls, and 29 healthy controls. The gene discussed is NEFL; the disease is amyotrophic lateral sclerosis.