To examine whether the Src kinase is involved in regulation of EphA2 expression, we used Src-specific siRNA to knock Src down in PTEN-deficient cells, including MCF10A-KO1 and MCF10A-KO2 cells, HCC1937 (breast cancer), and SPAC-1-L (EC) cells and confirmed that suppressing Src expression can substantially reduce EphA2 expression across all tested cell lines (Fig. 7, D and E). The gene discussed is PTEN; the disease is breast carcinoma.